In addition to a supported flow cytometer, the FirePlex microRNA assay requires a vacuum manifold compatible with 96-well filter plates and a thermal shaker. Click the titles below to read more.
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Required Equipment
Vacuum Manifold for 96-well Filter Plates
The Firefly BioWorks' vacuum manifold is a cost-conscious alternative for sample filtration in a 96-well filter plate. The manifold is optimized for the efficient filtration of FirePlex® microRNA samples, but is applicable to other samples. Manifolds by other manufacturers are compatible with miRSelect, if vacuum can be regulated to between 3 and 5 inHg.
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Vacuum Manifold 96-well Filter Plate $30 |
Shaking Incubator
FirePlex® miRSelect microRNA assay requires three microplate shaking steps (FirePlex® miRSelect Protocol). Although a dedicated thermal shaker is ideal, a shaking incubator or a stand-alone shaker inside of an incubator or warm-room is also acceptable. Sample thermal shakers and incubators compatible with the FirePlex® miRSelect assay include:
Talboys Incubating Microplate Shaker
LabNet VorTemp 56 Shaking Incubator
VWR Symphony Incubating Microplate Shaker
Biomega MultiTherm Shaker
Supported Cytometers
(Firefly BioWorks does not distribute flow cytometers)
Firefly particles are designed to be read on cytometers that have a blue (488nm) laser with green, yellow, and red detectors. The benchtop cytometers that FirePlex® miRSelect has been optimized to work with include:
FirePlex® miRSelect has also demonstrated multiplexed detection using larger core facility machines. Our products are approved for use with the following machines:
For other machine configurations, please contact us!