Does the Firefly microRNA Assay distinguish different isoforms of the same microRNA?

The Firefly microRNA Assay detects target isoforms of + or - one base with the same probe at about 50% efficiency. For differences of more than one base, a new probe is required. This is ideal because, in general, researchers wish to pool all similar isoforms and look for how they change together. However, if you would like to distinguish similar isoforms, we can design custom probes to allow for that. Just contact technical support and request further information.