How does the Firefly microRNA detection limit compare to my RNA-seq data?

Because of the technical differences in the assays, there is not a perfect correlation between read numbers from RNA-seq data and detection limits for the Firefly MicroRNA Assay. For microRNAs with over 2000 reads, we routinely have very robust signal. We can often detect targets with as few as 50 reads. However, there is considerable variability from target to target for those sequences with less than 2000 reads.