What’s the best way to purify microRNAs for use with the Firefly MicroRNA Assay?

We highly recommend the Qiagen miRNeasy product line of kits for cells, tissue, serum, plasma, and FFPE tissues.Trizol extraction also works well.

The Norgen BioTek kit works but gives 3-5 fold lower signal than Qiagen and is therefore not recommended for low RNA content samples. Qiagen RNeasy Total RNA extraction kits preferentially select for RNAs longer than 200bp and have not been validated with our assay.

IMPORTANT NOTE: Life Technologies/Ambion miRVana kit is incompatible with our particles and cannot be used.