To use the example files:
(1) Download “cytometer_data.fcs” and “product_bc20131017.plx” [here - 11MB ZIP file]
(2) Unzip the downloaded file.
(3) Start Firefly™ Analysis Workbench
(4) Select File → Import PLX and browse to the folder where you unzipped product_bc20131017.plx (often Downloads)
(5) Select File → Import FCS and browse to the folder where you unzipped cytometer_data.fcs (often Downloads)
(6) When it prompts for a barcode, enter bc20131017
(7) Set the negatives to be B11-B12, D11-D12 and F5-F10
(8) Explore creating experiments, generating heatmaps and sample/probe charts
Example output is shown below.