Bargraph

The bargraphs apply to the selected experiment, and show all the probe values in the selected sample(s), as well as the value of the selected probe(s) in all the samples.   They might be thought of as horizontal or vertical cross-sections of the heatmap.

An example chart for a single sample and for two samples is shown below.
Single sample
Two samples
image 1 sample
image 2 samples


An example chart  of a single probe and for two probes is shown below.  The probe chart includes the limit of detection (aka minimum detected dose) of each of the probes.
Single probe
Two probes
image 1 probe
image 2 probes

Bar are colored yellow if there is only a single probe or sample involved.   If multiple samples are being compared, there is one color for each sample, and similarly for the probe plot.  If a bar is below the limit of detection in the probe plot it is colored gray.  If the bar is below the bottom of the standard curve or above the top of the standard curve and cannot be interpolated, the bar is transparent, and is sometimes referred to as a "ghost" bar to indicate that data was measured but could not be transformed using the standard curve.

There are two presentation modes, "bar" presentation and "box and whisker" presentation.
In bar presentation, the bar extends from the horizontal axis to the mean of the data, and the error bars represent the 95% confidence interval of the estimate of the mean.   The statistics for a particular probe and sample are gathered from all the particles found in that sample that carry the barcode of that probe, typically several dozen particles.

In "box and whisker" presentation, the bar extends from the 25th percentile to the 75th percentile of the particle data values.   The whiskers extend from the 25th percentile down by 1.5 interquartile ranges (IQRs), and up from the 75th percentile by 1.5 IQRs.  

The values in the chart derive from the data at the end of the data processing pipeline

The sample and probe names are listed along the respective X axes.  If there are too many names to fit on the axis, they are down-sampled to make them fit.

Controls

See Also

Help Front Page
Limit of Detection
Standard Curve