The bargraphs apply to the selected experiment, and show all the
probe values in the selected sample(s), as well as the value of the
selected probe(s) in all the samples. They might be
thought of as horizontal or vertical cross-sections of the heatmap.
An example chart for a single sample and for two samples is shown
An example chart of a single probe and for two probes is shown
below. The probe chart includes the limit of detection (aka minimum
detected dose) of each of the probes.
Bar are colored yellow if there is only a single probe or sample
involved. If multiple samples are being compared, there
is one color for each sample, and similarly for the probe
plot. If a bar is below the limit of detection in the probe
plot it is colored gray. If the bar is below the bottom of the
standard curve or above the top of the standard curve and cannot be
interpolated, the bar is transparent, and is sometimes referred to
as a "ghost" bar to indicate that data was measured but could not be
transformed using the standard curve.
There are two presentation modes, "bar" presentation and "box and
In bar presentation, the bar extends from the horizontal axis to the
mean of the data, and the error bars represent the 95% confidence interval of the estimate
of the mean. The statistics for a particular probe and
sample are gathered from all the particles found in that sample that
carry the barcode of that probe, typically several dozen particles.
In "box and whisker" presentation, the bar extends from the 25th
percentile to the 75th percentile of the particle data
values. The whiskers extend from the 25th percentile
down by 1.5 interquartile ranges (IQRs), and up from the 75th
percentile by 1.5 IQRs.
The values in the chart derive from the data at the end of the data
The sample and probe names are listed along the respective X
axes. If there are too many names to fit on the axis, they are
down-sampled to make them fit.
- The charts can be zoomed and panned.
- Samples and probes can be selected.
- The data can be converted to log scale with the checkbox.
- Switch between "bar" presentation and "box and whiskers"
- The individual points going into each measurement can be
charted, using the checkbox.
- The count of particles going in to each measurementcharted can
be using the checkbox.
- The minimum detectable dose
is shown as a horizontal line on the probe chart, but it can be
- The upper and lower bounds of the plots can be set explicitly
using the numeric fields ymin and ymax
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Limit of Detection