The export button can be used to save either a binary workspace or a commaseparatedvalues (CSV) format file.
The binary workspace can be reloaded for further analysis in FirePlex Analysis Workbench.
The CSV file can be read into R, Matlab, Excel, or other data analysis program for further processing.
The exported file contains a matrix of data. The format of the matrix is shown below.
The samples are in the rows (A01, A02, A03... in the figure).
The probes are in the columns (p1, p2, p3, p4... in the figure).
By default, only probes and samples which are not marked as hidden are exported. If desired, the hidden probes and samples can also be exported using the checkbox on the export options form.
Each probe is represented by several potential statistics, including mean, standard error of the mean, interparticle standard deviation, interparticle coefficient of variation, and particle count. These are represented as the suffices a,b,c... on the probe names in the figure. The sample/probe matrix for each statistic is contiguous, so all the signal values are together, all the CoV values are together, etc.
The signals in the matrix represent the values at the end of the signal processing pipeline, and are potentially blank subtracted, negative subtracted, and normalized, depending on user choices.
If only the signal values and not the std. deviation, CoV etc are desired, select "simple" on the export popup form. The column headers indicated as "p1a" in the table below, will be written either as a single cell "p1asignal" or as two cells, with "signal" in the top row and "p1a" in the second row, according to the popup item "combine headers". Downstream programs such as Excel or R should skip either one row or two rows when they read the data.
Three dummy columns are added, including a negative column to represent whether the sample in a row is a negative sample, a probes column giving the total number of probes detected in a particular sample, and a particles column, giving the total number of particles detected in that sample (over all probes).
Three dummy rows are added. The first is a blank row to indicate for each probe whether it was used as a blank probe, the second one is a normal row, to indicate whether it was used as a normalization probe. The third row is the minimum detectable dose of that probe.
One matrix is exported for each experiment. All matrices can be written to one file, or each to a separate file, according to the export popup.
p1a 
p2a 
p3a 
p4a 
p1b 
p2b 
p3b 
p4b 
p1c 
p2c 
p3c 
p4c 
negatives 
probes 
particles 

A01 
0 
np 
N 

A02 
0 
np  N  
A03 
0 
np  N  
A04 
0 
np  N  
A05 
0 
np  N  
B01 
0 
np  N  
B02 
0 
np  N  
B03 
0 
np  N  
B04 
1 
np  N  
B05 
1 
np  N  
blank 
1 
1 
1 

normal 
1 
1 
1 

MDD 
z1 
z2  z3  z4  z1 
z2 
z3 
z4 
z1 
z2 
z3 
z4 