Flow cytometer data


When reading barcoded particle data from a flow cytometer, there are three stages of data conversion
Process of reading flow cytometry data
The decoded particles can be imagined as an elevation map of the data, where the longitude is the sample ID, the latitude is the probe ID, and elevation is the number of particles detected for that combination of sample and probe.   To simplify data visualization, the particle statistics for each sample/probe combination are collapsed into a mean signal (and standard deviation).  The result is a signal matrix, with the rows corresponding to the samples, the columns corresponding to the probes, and the matrix entries corresponding to the mean signal for that sample and probe combination.

Cytometer-specific configurations have been created for channel assignment and particle calling.
See
http://www.abcam.com/protocols/flow-cytometry-protocols-for-multiplex-mirna-assays

for the most up-to-date list.

See Also

Data processing
Data statistics
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