Plate

A plate is a visual representation of a microtiter  plate, primarily used to select, group and separate samples. 96-well, 384-well and 1536-well plates are supported.

Samples on a plate may be auto-named using an Excel template.   Templates can be downloaded at the FirePlex software website.

FCS samples on a plate go through particle analysis together using a single barcode.   If multiple different barcodes are mixed in the same plate, it is imperative to break the plate down into its sub-plates , otherwise the results will be unreliable.   For instance, if one barcode is a 70 plex and the other is a 20 plex, there is no appropriate basis for analyzing the samples from each barcode together. 

Naming conventions

The row names on a 96-well plate are A-H and the column names are 1-12.
The row names on a 384-well plate are A-P and the column names are 1-24.
The row names on a 1536-well plate are A-Z,27-32 and the column names are 1-48.  When samples are loaded from an imager, it is often helpful to use
"order by column" or "order by row" to normalize sample order, as the file order on disk is unpredictable.


See Also

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