well, patientId, condition A01, pid1, pre-treatment A02, pid2, pre-treatment A03, pid3, pre-treatment A04, pid4, pre-treatment B01, pid5, post-treatment B02, pid6, post-treatment B03, pid7, post-treatment B04, pid8, post-treatment C01, pid9, normal C02, pid10, normal C03, pid11, normal C04, pid12, normal C05, aneg, NA C06, aneg, NA C07, apos, NA C08, apos, NAThe header rows specifies that there are three columns in the file. The first two columns are always the well location and sample name. Any number of other columns may be named, which will become named variables. The variables can be used to pool samples across replicates, or as factors for Anova analysis. If the fields contain commas, they must be enclosed in quotes, e.g. "Sample1,day1"
NRdefines the number of rows being described, and
NCthe number of columns. The
namefollowing the semicolon identifies what is being defined. The special name
SampleNamemeans that the block describes the names to be assigned to samples. Another special name is
Dilution, where the value is the dilution assigned to the sample. A positive dilution, e.g. 0.25, means the sample is a positive control and the dilution is relative to the highest standard concentration. A negative dilution, e.g. -0.25, means the sample has been diluted 4-fold before being aliquoted into the well, and the extracted protein concentration will be adjusted upward by a factor of 4 in charts and export files.
PlateLayout(8,6);variablecould define the layout of a block of 8 rows and 6 columns.
||The column (list format) used to identify the well. Well is always the first column in list form. In block form the Well is implicit from position.|
||The block (table format) or
column (list format) used to define the short sample
names shown in the Experiment table and in export
files. SampleName is always the second column
in list form.
||A positive dilution (e.g.
0.3333) means the sample is a positive control and the
dilution relative to the highest standard concentration is
A negative dilution (e.g. -0.25) means that the in-well concentration of the sample is 1:4 diluted relative to the original stock.
A zero dilution means that the sample is a negative control, e.g. water or diluent without sample.
|Special keywords used for
Discovery panels, should not be used.
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